Table 1.
Phenotypes of MBP83–99-specific T cells established from MS patients
| T cells | FOXP3 | CD69 | CD25 | CD127 | HLA-DR | GITR | CD122 | CD132 | CD62L | CTLA-4 |
| 1D2 | 49.3 | 32.1 | 512.1 | 3.2 | 744.8 | 6.5 | 18.5 | 186.3 | 19.2 | 101.7 |
| 1C6#6 | 21.6 | 35.6 | 544.8 | 1.9 | 703.4 | 9.1 | 6.8 | 121.5 | 19.7 | 133.5 |
| 1F5#9 | 20.3 | 11.4 | 512.9 | 4.1 | 706.3 | 5.2 | 20.3 | 100.3 | 18.3 | 201.1 |
| 1B10 | 21.7 | 20.1 | 532.5 | 3.7 | 711.1 | 2.1 | 15.2 | 89.7 | 12.1 | 127.6 |
| 1C7 | 33.1 | 21.8 | 576.6 | 2.4 | 678.5 | 3.4 | 19.1 | 179.3 | 23.4 | 196.4 |
| 2D7 | 35.5 | 33.6 | 531.1 | 3.6 | 716.8 | 5.8 | 13.6 | 111.5 | 17.3 | 157.1 |
| G2 | 37.8 | 39.5 | 517.4 | 5.3 | 732.2 | 3.6 | 11.2 | 99.3 | 21.2 | 121.3 |
| 2C6 | 24.4 | 25.8 | 542.1 | 4.5 | 736.5 | 4.3 | 11.9 | 102.1 | 18.3 | 172.9 |
| 2E3 | 41.2 | 29.1 | 432.7 | 5.7 | 721.6 | 9.3 | 21.3 | 109.8 | 24.8 | 106.5 |
| F1 | 44.5 | 34.2 | 444.7 | 5.1 | 689.9 | 6.9 | 9.7 | 97.2 | 22.3 | 132.1 |
| Meana | 32.9 ± 10.4 | 28.3 ± 8.5 | 514.7 ± 44.3 | 4.0 ± 1.2 | 714.1 ± 20.7 | 5.6 ± 2.4 | 14.8 ± 4.9 | 120.0 ± 34.4 | 19.7 ± 3.6 | 145.2 ± 35.3 |
| G5 | 1.3 | 10.7 | 501.2 | 4.7 | 735.1 | 6.3 | 16.5 | 101.6 | 8.2 | 38.3 |
| E3 | 2.1 | 15.6 | 512.9 | 3.3 | 763.5 | 6.9 | 7.2 | 167.3 | 10.6 | 71.2 |
| 1C4 | 5.8 | 31.2 | 397.9 | 5.8 | 699.1 | 7.6 | 9.1 | 97.7 | 3.5 | 33.7 |
| A11#2 | 15.1 | 14.3 | 432.2 | 4.9 | 756.3 | 3.9 | 10.7 | 94.2 | 8.9 | 89.5 |
| 2E5 | 3.1 | 19.5 | 512.7 | 3.7 | 712.2 | 5.1 | 9.3 | 171.4 | 4.7 | 73.3 |
| B9 | 2.7 | 33.7 | 532.2 | 2.9 | 756.4 | 2.3 | 15.3 | 103.5 | 7.6 | 46.8 |
| 2D5 | 3.1 | 25.6 | 576.6 | 2.0 | 788.2 | 7.5 | 7.6 | 98.2 | 5.2 | 47.6 |
| 1F8 | 3.2 | 37.5 | 478.2 | 5.5 | 725.3 | 3.9 | 11.3 | 168.3 | 6.7 | 64.8 |
| 1F4 | 10.8 | 22.3 | 279.7 | 6.7 | 798.6 | 4.2 | 8.8 | 189.1 | 5.6 | 42.6 |
| 1G5 | 7.5 | 31.4 | 532.1 | 5.4 | 709.8 | 4.6 | 7.5 | 135.2 | 11.2 | 34.1 |
| Meanb | 5.5 ± 4.4 | 24.2 ± 9.1 | 475.6 ± 85.8 | 4.5 ± 1.5 | 744.5 ± 33.7 | 5.2 ± 1.8 | 10.3 ± 3.2 | 132.7 ± 37.8 | 7.2 ± 2.5 | 54.2 ± 19.2 |
Mean florescence intensity (MFI) was determined by FACSCalibur (BD Bioscience) and the data analysis software (FCS Express, De Novo software). Data are presented for all clones tested for phenotypes. First, all MBP83–99-specific T-cell clones were analyzed for FOXP3 expression. Subsequently, 10 clones were selected for each group representing polarized FOXP3 expression and undergone detailed phenotypic analysis.
a
Mean ± SD of MFI from FOXP3high group.
b
Mean ± SD of MFI from FOXP3low group.