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. 2009 Oct 30;21(12):1351–1361. doi: 10.1093/intimm/dxp106

Fig. 4.

Fig. 4.

Requirement of hnRNP-K for activation of the IL-2 promoter. (A) Jurkat cells were transfected with IL-2–Luciferase reporter construct and siRNA expression vector against hnRNP-K (siK, open bar) or control vector (vector, closed bar). Twenty-four hours later, cells were stimulated with anti-TCR antibody (αTCR) or PMA plus ionomycin (P + I). Cell were harvested 18 h later and used for luciferase assay. Error bars show SD n = 3; *P < 0.005. (B) Jurkat Tag cells were co-transfected with expression vector for HA-tagged hnRNP-K (HA-K, closed bar) or control vector (vector, open bar). Twenty-four hours after transfection, cells were stimulated and analyzed as described in (A); *P < 0.005. (C) Jurkat cells were transfected with NF-κB-luciferase, NF-AT-luciferase, AP-1 luciferase or HSP-90α luciferase reporter constructs with siRNA expression construct for hnRNP-K (siK, closed bar) or control vector (vector, open bar). Transfected cells were stimulated (α-TCR) or left unstimulated (unstim.) and analyzed for luciferase activity; *P < 0.005, **P < 0.05.