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. 2009 Nov 30;4(11):e8017. doi: 10.1371/journal.pone.0008017

Figure 4. Oligomeric status of MsDps2ΔN15 and its DNA bound complex.

Figure 4

A) The N-terminal deleted protein MsDps2ΔN15 is a dodecamer like the full-length protein. Lane 1 and 2 are the markers, BSA and Ferritin respectively, on a 10% native gel; Lane 3 is MsDps2; while lane 4 shows MsDps2ΔN15. B) MsDps2ΔN15 protein in 20 mM Tris-HCl (pH 7.9), 200 mM NaCl, purified by DE52 ion-exchange chromatography was checked on a 0.8% agarose gel for bound DNA.Lane 1: Free plasmid DNA; Lane 2: DNA+ MsDps2NΔ15 (1∶104 molar ratio).