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. 2009 Dec 4;5(12):e1000680. doi: 10.1371/journal.ppat.1000680

Figure 2. Restriction fragment length polymorphism assay for switching at vlsE.

Figure 2

A portion of the vlsE expression site containing the variable regions was amplified using primers B248 and B249 to give a product of 776 bp. PCR reactions were performed on B. burgdorferi grown from ear biopsies taken at day 21 and the products were digested with HphI and run on a 1.2% agarose gel in TAE buffer at 75V for 1.5 hours and stained with ethidium bromide (see Materials and Methods). Wild-type B. burgdorferi B31-5A4 recovered following infection of a C3H/HeN mouse was used as a template in lanes 1 and 2. An unswitched template (not exposed to mouse infection) is shown in lanes 3 and 4. PCR products from rep2 and mutS2/1 DNA templates are found in lanes 5 & 6, and 7 & 8 respectively. M denotes a 100bp molecular weight marker.