Figure 3. Number of switched vlsE clones in SCID C3H/HeN mice.

Sequencing of the cloned PCR product of the vlsE variable regions using primer pJET1.2/forward was performed on 10 clones from each tissue type culture for each genotype (see Materials and Methods). The y-axis denotes the number of clones out of ten that contained templated nucleotide changes in variable regions 1–6 (switches) and the x-axis denotes the tissue type. The P-values above the bars indicate the level of significance of the difference between the wild-type and mutant samples, calculated using Fisher's Exact test.