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. Author manuscript; available in PMC: 2010 Sep 29.
Published in final edited form as: Circulation. 2009 Sep 14;120(13):1255–1265. doi: 10.1161/CIRCULATIONAHA.109.873695

Figure 3. Immunodepletion of platelets accelerates TGN.

Figure 3

(A) TGN (LPS + αGBM) was induced in wild-type mice and renal tissue was harvested 4hrs later from these mice and untreated wild-type mice (normal) to immunohistochemically assess platelet accumulation using antibody that recognizes the αIIb subunit of αIIbβ3 on platelets. (B-F) WT mice were intravenously injected with anti-GPIbα (40μg/mice) or control IgG (Con) 12 hrs prior to TGN induction. Glomerular PAS and fibrin deposition (PTAH staining) were significantly increased (B) and glomerular CD34 expression was reduced (C) in platelet-depleted mice in comparison with controls. The hematuria grade (D) was greater as were biochemical markers for renal function (BUN, Cr) and general organ damage (LDH) (E). The number of neutrophils (PMNs) per glomerulus (F) was also increased in platelet depleted mice. Data are the mean ± SEM. *P<0.05 compared with control IgG group. Bar: 30 μm