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. Author manuscript; available in PMC: 2009 Nov 20.
Published in final edited form as: Nat Immunol. 2009 Mar 8;10(4):437–443. doi: 10.1038/ni.1721

Figure 4. Identification of C/EBPδ-direct targets.

Figure 4

a, Macrophages from wild-type mice were stimulated for 6 h with 10 ng/ml LPS. Cells were harvested and processed for immunoprecipitation with polyclonal antibodies against C/EBPδ. The binding of immunoprecipitated C/EBPδ to the promoters of target genes was detected using the Affymetrix GeneChip Mouse Promoter 1.0R Array. Averaged and normalized C/EBPδ binding across indicated promoters are shown for LPS-stimulated (red line) and unstimulated (blue line) cells. Arrow, transcription start site. Data represent the average of two independent experimental values.

b, Macrophages from wild-type and Cebpd-/- mice were stimulated with 1ng/ml LPS for the indicated time periods. Four hours after LPS stimulation cells were harvested and indicated mRNA transcripts were measured by quantitative real-time RT-PCR. Data points represent the average of three independent experimental values and error bars indicate ± the standard error.