Skip to main content
. Author manuscript; available in PMC: 2010 Oct 16.
Published in final edited form as: Cell. 2009 Oct 16;139(2):416–427. doi: 10.1016/j.cell.2009.08.035

Figure 3. c061 labels six DA neurons that innervate the MB.

Figure 3

A. Projection view of a c061;uas-CD8::GFP brain. Filled yellow arrows mark the heel region of the MB resembling that in TH-GAL4 labeled brains (Figure S4A). B. Combining MBGAL80 with c061;uas-CD8::GFP eliminates MB expression and reveals neurons that innervate the heel of the MB (filled yellow arrows). Also see Movie S1 and Movie S2. C. Combining THGAL80 with c061;uas-CD8::GFP eliminates expression in the neurons innervating the region between the MB lobes and MB heel (hollow arrows) but leaves expression elsewhere intact. D. Projection view of a c061;MBGAL80/THGAL80;uas-CD8::GFP brain. THGAL80 removes expression from the neurons innervating the dorsal protocerebrum and MB heel (hollow arrows) and leaves expression in the fan-shaped body and elsewhere intact. Scale bar represents 20 µm. E. Higher magnification single confocal section views of the MB heel and peduncle region in a c061; uas-CD8::GFP brain. Top panel shows innervation of the MB heel. Bottom panel, innervation in the base of the peduncle. Inset, section through the peduncle showing zones occupied by the αβ,α´β´ and γ MB neurons. The MB is co-labeled with MB-DsRED. F. Confocal section through a c061;MBGAL80;uas-CD8::GFP brain at the level of the MB calyx (outlined). GFP (green) labels 3 cell bodies at the side of the calyx and 5 more lateral cell bodies. Counter staining with anti-TH antibody (red) labels 12 cell bodies in the PPL1 cluster and 3 of them overlap (merge, yellow) with c061;MBGAL80 driven GFP. Scale bar represents 10 µm. G. Confocal section through a PPL1 cluster in a c061;MBGAL80/THGAL80;uas-CD8::GFP brain. GFP (green) labels 5 cell bodies and none overlap with anti-TH staining. Scale bar represents 10 µm.