Figure 3.

Upregulation of survivin by T138067 is associated with the modulation of the phosphorylation of Akt and MAPK molecules. A. T138067 increases Akt phosphorylation. B. T138067 increases Erk1/2 phosphorylation. C. T138067 decreases p38 MAPK phosphorylation but shows no effect on the phosphorylation of ErbB2. MCF-7 cells were treated with T138067 over the time indicated. Cells were then lysed and analyzed for phosphorylation of Akt, Erk1/2, p38 MAPK and ErbB-2 by western blots with the phospho-Akt-specific, phospho-Erk1/2-specific, phospho-p38-specific and phospho-ErbB2-specific antibodies. Total Akt and Erk1/2 and p38 proteins were monitored as internal controls.