Figure 5.

Inhibition of T138067-induced activation of Akt and Erk1/2 decreases T138067-mediated induction of survivin expression. A. MCF-7 breast cancer cells were treated with T138067 in the presence and absence of PI3K inhibitor LY294002. Cells were lysed 24 hours after treatment and analyzed for survivin expression by western blots as shown. B. MCF-7 cells were treated with T138067 in the presence and absence of MEK inhibitors, PD98059 and U0126 as shown. Cells were lysed 24 hours after treatment and analyzed for survivin expression by western blots. β-Action was used as internal controls for protein loading for both A and B.