Figure 9.

Resveratrol inhibits survivin expression and significantly enhances the effectiveness of T138067 to induce breast cancer cell death. A. ZR-75 breast cancer cells were treated with T138067 at different concentrations for 24 hours as shown. Cells were then lysed and analyzed by western blots to determine survivin expression. β-Action is an internal control for protein loading. B. ZR-75 cells were treated with T138067 or resveratrol alone or in combination. For combination treatment, cells were treated sequentially with resveratrol (30 μM) for 24 hours, followed by T138067 (20 nM) for 24 hours. Pictures were taken at 7 days after Resveratrol treatment.