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SCE jumping in P. aeruginosa. Schematic diagram illustrates high-density mutagenesis of a targeted chromosomal region. Cloned regions of interest are mutagenized in vitro and introduced into P. aeruginosa. Presence of the I-SceI enzyme prevents cointegrate formation. The mating mixture is plated onto the appropriate antibiotics, and growth selects against insertions in regions containing essential genes.
