. 2000 Aug 29;97(18):10191–10196. doi: 10.1073/pnas.97.18.10191

Table 1.

Allelic replacement of pyrF by using SCE jumping

Recipient strain	−Ura in minimal		+Ura in minimal
Recipient strain	Sm^rGm^rCb^r	Suc^rFOA^r	Sm^rGm^rCb^r	Suc^rFOA^r
SW658	1.7 × 10⁴	0/25	3.4 × 10⁴	12/25
SWSce	0	0	0.9 × 10⁴	28/28

Suicide plasmid pSWΔFGm was transferred conjugally from E. coli donor into P. aeruginosa recipients SWSce (contains I-SceI-expressing plasmid) or SW658 (contains parent plasmid pJB658). After overnight mating at 37°C, transconjugants were plated onto minimal medium containing Sm, Gm, and Cb with or without Ura. Data are recorded as number of Sm^r, Gm^r, and Cb^r isolates per 10⁸ recipients. Representative isolates were tested for Suc and FOA sensitivity.