Table 1. Transposon mutants utilized in this study.

Mutant	Replicon	Insertion Sitea	Insertion Ratiob	Gene	Predicted Product	Infectivity	Results, RFLP Analysis of vlsE recombination
T11P01A01	Chrm	22339	0.67	BB0023	junction DNA helicase, subunit A (RuvA)	Reduced	Reduced
T03TC051	Chrm	21916	0.18	BB0022	junction DNA helicase, subunit B (RuvB)	Reduced	Reduced
T05P01C02	Chrm	841133	0.73	BB0797	DNA mismatch repair protein (MutS)	Reduced	Reduced
T10P01D06	Chrm	841754	0.97	BB0797	DNA mismatch repair protein (MutS)	Normal	Normal
T10P01G11	Chrm	632960	0.24	BB0607	rep helicase, single-stranded DNA-dependent ATPase (Rep)	Normal	Normal
MG065	Chrm	96742	0.09	BB0098	recombination and DNA strand exchange inhibitor protein (MutS-II)	Noninfectious	NDc
T08P01E02	lp25	20092	0.33	BBE29	adenine specific DNA methyltransferase, authentic frameshift	Normal	Normal
T11P01F09	lp25	20047	0.30	BBE29	adenine specific DNA methyltransferase, authentic frameshift	Normal	ND
T09P01G01	lp17	12892	0.88	BBD20	transposase-like protein, authentic frameshift	Normal	Normal

The plasmid content of the mutants was the same as that of the parent strain 5A18NP1 (lp28-4⁻, lp56⁻) for all of the clones except for T11P01A01 and T11P01F09, which were also cp9⁻.

^aCoordinates of transposon insertion in the indicated replicon. Chrm = chromosome.

^bNumber of nucleotides(beginning of the open reading frame to the transposon insertion site)/Number of nucleotides(open reading frame).

^cNot determined.