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. 2009 Dec 1;4(12):e8111. doi: 10.1371/journal.pone.0008111

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Neves-Costa et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Diagram of the telomeric reporter genes in strain YTI312 (adapted from [35] oval: centromere, open boxes:URA3 and ADE2 reporter genes, small ovals: telomere. B. Silencing of the telomeric ADE2 (YTI312, [35]) in the presence but not in the absence of Fun30 (YTI312;fun30Δ). C. Analysis of role of Fun30 in telomeric silencing of a URA3 reporter by serial dilution assay on plates without or with 5-FOA. D. Representation of the rDNA structure of strain JS306 (adapted from [37]). E. Analysis of role of Fun30 in silencing of HIS3 or MET15 reporter genes within the rDNA locus by serial dilution assay on plates with complete synthetic media or media lacking either histidine or methionine.