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. 2009 Dec 1;4(12):e8111. doi: 10.1371/journal.pone.0008111

Figure 6. Fun30 binds at the silent HMR locus.

Figure 6

A. Validation of the specificity of the affinity purified Fun30 antibody for chromatin immunoprecipitation (ChIP). Control (wt) and fun30Δ cells were analyzed by ChIP with Fun30 antibody. Immunoprecipitated DNA was quantified at the a1 locus using quantitative PCR, corrected for background signal using the IgG control, and normalized relative to input. Shown is a representative result of two repeat experiments. B. upper panel: ChIP analysis of Fun30 occupancy across the HMR barrier. Error bars are standard deviation of the two biological replicates of this experiment. Lower panel: Diagram of the HMR barrier region. Arrows indicate the orientation of specific genes in the region. Small bars underneath indicate the relative position of qPCR probes used in ChIP analyses. C. Fun30 occupancy near the telomere VIR.