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. 2009 Dec 1;4(12):e8111. doi: 10.1371/journal.pone.0008111

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Neves-Costa et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Silencing of the ADE2 reporter in YLS586; fun30Δ is restored by expressing wildtype Fun30 in trans, but not by a Fun30 ATPase mutant or by a control vector expressing LacZ. Expression of Fun30 with the putative CUE motif deleted does not restore silencing as monitored by red pigment formation. Expression was in galactose at 30°C, driven by the galactose-inducible promoter of GAL1. B. Immunoblot analysis of the Fun30 protein in trans-expression with anti-V5 antibody. An immunoblot for histone H3 served as protein loading control. V5-tagged Fun30 and indicated mutants thereof were expressed in trans in a galactose- or glucose dependent manner in Fun30 containing strain JRY7385 from multicopy plasmids, the control vector expresses V5-tagged beta-galactosidase. C. Cells from (B) were serially diluted 10-fold, spotted on media with galactose or with glucose and grown for 3 days at 30°C.