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NF-κB p65 DNA-binding activity is increased in NSCLC cells with altered p53. (a,b) 5 µg of nuclear lysates of the indicated mouse (a) or human (b) cell lines were analyzed for p65 DNA-binding activity by an ELISA assay (see Methods). Cells harboring WT p53 are indicated. As a positive control of p65 activity, LKR13 cells (a) were stimulated with TNF for 30 minutes prior to lysis.
