Fig. 1. IL-1RI is required for alum-induced neutrophilia.

Peripheral blood cells of BL/6 and IL-1RI^−/− mice were harvested after immunization (1–8 d) with NP₈-CGG/alum. Neutrophils, inflammatory monocytes, and eosinophils were enumerated by flow cytometry (Supplemental Fig. 1). The mean (± SEM) numbers of cells/ml of blood from BL/6 (●; n=4–7) and IL-1RI^−/− (○; d 1 and 2, n=2, others, n=4–5) mice are shown. Significant differences from naïve controls are indicated for BL/6 (*, P ≤ 0.05; **, P ≤ 0.01) and IL-1RI^−/− (^†, P ≤ 0.05;^††, P ≤ 0.01) mice.