Fig. 4. Robust inflammatory responses in IL-1RI^−/− mice.

BM cells of BL/6 mice and IL-1RI^−/− mice were harvested after immunization (1–8 d) and labeled with mAbs to identify eosinophils (Supplemental Fig. 1) and pre-B cells [ref (10)]. Average numbers (± SEM) of eosinophils (A) and pre-B cells (B) from BL/6 (●) and IL-1RI^−/− (○) mice are shown (n=3–11, each point). Significant differences from naïve controls are indicated for BL/6 (*, P ≤ 0.05; **, P ≤ 0.01) and IL-1RI^−/− (^†, P ≤ 0.05; ^††, P ≤ 0.01) mice. (C) NP₁₆-BSA binding λ⁺ and κ⁺ serum antibody was determined in BL/6 (closed symbols) and IL-1RI^−/− (open) mice (n=2, each point) before and after immunization with 60 μg NP₈-CGG/alum. NP-specific serum λ (circles) and κ (squares) antibodies were quantified (mean±SD) by ELISA.