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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Clin Immunol. 2009 Jun 24;132(3):371–384. doi: 10.1016/j.clim.2009.05.016

Figure 4.

Figure 4

VEGF regulates the expression of specific chemokine receptors (A–D), chemokines (A), and cytokines (A–C) in lung mDC. (A, B) cDNA from sorted lung mDC was obtained as described in Materials and Methods and analyzed for the selected markers using PCR with corresponding primer’s set. Lung VEGF expression regulated local mDC expression of selected chemokine receptors, chemokines, and regulatory cytokines. (B) Upregulation of IL-12p40 expression in tg lung mDC as analyzed by a quantative real time RT-PCR. (C) Sorted lung mDC were subjected to a protein lysis buffer. Fifteen µg of cellular protein were subjected to a Western blot analysis. Lung VEGF expression induced an upregulation in CCR5 and IL-1β expression in lung mDC. (D) Increased expression of CCR7 (solid line over transparent line which shows rat IgG2a isotype control stain) on sorted lung mDC obtained from VEGF tg mice as compared to WT cell marker expression analyzed by flow cytometry. (A–D) Mice were placed on DOX water for 7 days.