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VEGF-induced increase of in vitro Ag uptake by lung mDC. Lung mDC were sorted using CD11c and MHCII marker expression and subjected to the in vitro cultures with or without OVA-FITC as described in Materials and Methods. Cells were further analyzed by flow cytometry. The shown are absolute and relative numbers of FITC+ mDC among 3000 analyzed cells for each setting in one out of two experiments. Note an increased VEGF tg mDC ability to uptake OVA-FITC at low Ag doses.
