Table 3.
Vaginal application of FSL-1 in mice induced a specific cytokine profile.
| 6 h Post FSL-1 application (pg/lavage ± SEM) | ||
|---|---|---|
| Cytokinea | FSL-1 | DPBS vehicle |
| IL-1α | 1083.2 ± 143.5b | 527.0 ± 65.7 |
| IL-1β | 1157.0 ± 320.2c | 169.2 ± 46.3 |
| IL-2 | 34.8 ± 5.2d | BDL(3.9)e |
| IL-6 | 418.5 ± 94.1b | 35.4 ± 8.5 |
| IL-12(p40) | 104.2 ± 10.5d | 44.9 ± 3.8 |
| IL-12(p70) | 64.0 ± 19.1c | 17.1 ± 3.1 |
| G-CSF | 5157.0 ± 1095.0c | 2208.0 ± 433.1 |
| GM-CSF | 198.6 ± 44.6b | 22.6 ± 8.7 |
| IFNα | 30.3 ± 4.4d | 0.7 ± 0.6 |
| IFNβ | 445.3 ± 93.2b | 13.8 ± 3.5 |
| IFNγ | 76.8 ± 18.8c | BDL(43.1) |
| MIP-1α | 2770.7 ± 484.0b | 440.0 ± 127.0 |
| MIP-1β | 4140.0 ± 570.5c | 2232.0 ± 218.5 |
| TNF-α | 1109.6 ± 212.7b | 232.6 ± 71.6 |
a The indicated cytokines were found to be induced by FSL-1 application by BioPlex or ELISA as indicated in the methods. Data are presented as mean ± SEM of five replicates from two independent experiments.
b p < 0.01 compared to DPBS vehicle (Student's t-test).
c p < 0.05 compared to DPBS vehicle (Student's t-test).
d p < 0.001 compared to DPBS vehicle (Student's t-test).
e BDL; Below Detection Limit (for statistical comparisons with these cytokines the value for the lowest standard of each cytokine was utilized).