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. Author manuscript; available in PMC: 2009 Nov 20.
Published in final edited form as: Immunity. 2000 Jul;13(1):73–84. doi: 10.1016/s1074-7613(00)00009-1

Figure 6. Expression Pattern of Notch1 Target Genes in T Cells.

Figure 6

(A) RT-PCR analysis of the expression of Notch target genes in the AKR1 and AKR1010 thymomas and the 2B4.11 T cell hybridoma. Equivalent amounts of cDNA prepared from control and NotchIC expressing cells were serially diluted 5-fold and amplified using primers specific to the indicated genes. PCR products were analyzed by Southern blotting using specific probes.

(B) RT-PCR analysis of the expression of Notch target genes in DP thymocytes from NotchIC and littermate control mice. DP thymocytes were sorted from NotchIC and littermate control mice.

(C) RT-PCR analysis of the expression of Notch1 target genes in thymocyte subsets. Thymocyte subsets were sorted from B6 mice.

(D) RT-PCR analysis of the expression of Notch1 target genes in peripheral T cells. CD4+ and CD8+ T cells were sorted from peripheral lymph nodes of B6 mice.