Table 2.
Trace residues of the human Dishevelled DIX domain
| Dishevelled DIX trace analysis | Known single non-synonymous substitutions and their consequences | ||||||
|---|---|---|---|---|---|---|---|
| DIX trace residuea | CBb | SBb | CEb | SEb | Mutant | Characterization (genetic and/or biochemical) | Reference |
| Y8 | X | ||||||
| T15 | X | ||||||
| P16 | X | ||||||
| V19 | X | ||||||
| L30 | X | ||||||
| D32 | X | ||||||
| F33 | X | F40S (UAS-dsh8-65) c | Loss-of-function | [29] | |||
| K34 | X | ||||||
| K50 | X | DSH2 K58A d | Unable to bind actin | [28] | |||
| M52 | X | ||||||
| D53 | X | ||||||
| D55 | X | ||||||
| F56 | X | ||||||
| V58 | X | ||||||
| V59 | X | V66A (UAS-dsh8-19) c, e | Loss-of-function | [29] | |||
| K60 | X | DSH2 K68A e | Unable to homo-dimerize; cannot interact with lipids |
[11,28] | |||
| E61 | X | DSH2 E69Ae | Unable to homo-dimerize; cannot interact with lipids |
[11,28] | |||
| E62 | X | ||||||
| D66 | X | ||||||
| L70 | X | ||||||
| P71 | X | ||||||
| F73 | X | ||||||
| N74 | X | N80I (UAS-dsh8-80) c | Loss-of-function | [29] | |||
| G75 | X | ||||||
| V77 | X | ||||||
| V78 | X | ||||||
| S79 | X | ||||||
| W80 | X | ||||||
a numbering according to human Dishevelled DIX sequence
b CB, conserved and buried; SB, class-specific and buried; CE, conserved and exposed; SE, class-specific and exposed
c mutants of Drosophila melanogaster Dishevelled
d Dishevelled 2 actin-binding residue [28,30]
e Dishevelled 2 micelle-binding residue [30]