Dissociation between peripheral blood chimerism and tolerance to hindlimb composite tissue transplants: preferential localization of chimerism in donor bone

. Author manuscript; available in PMC: 2010 Sep 27.

Published in final edited form as: Transplantation. 2009 Sep 27;88(6):773–781. doi: 10.1097/TP.0b013e3181b47cfa

Multilineage Analysis^a

Group	n	% donor	% in donor (RT1 A^ab1+) lymphoid gate ^b
			T cells		B cells	NK cells	DC	Macrophage
			CD4	CD8	CD45RA	NK161	OX62	CD11b/c
A	18	34.3±14.3	36.9±15.7	26.3±12.9	25.7±15.1	17.8±8.1	6.5±5.7	24.3±11.8
B	12	30.7±6.6	29.9±16.9	24.8±15.1	43.4±13.8	16.1±8.3	11.6±14.0	20.6±11.5

A: Conditioned with tacrolimus/ALS and TBI from 600 to 300 cGy.

B: Conditioned with anti-αβ-TCR/tacrolimus/ALS and TBI from 400 to 300 cGy.

Multilineage typing was performed in mice between 2 and 3 mo after 2nd BMT.

Mean ± SD percentage of donor cells in donor lymphoid gate.