Figure 2.

Combined transcriptomic-proteomic analysis for identification of STAT1-dependent functions. (A) Outline of transcriptomic-proteomic approach coupled with functional analysis for the identification of STAT1 [Signal Transducer and Activator of Transcription 1]-dependent molecules and functions. (B) Expressional clustering of differentially expressed genes (92) and proteins (266) in untreated SCC61 STAT1 wild-type and knockdown tumours: red, up-regulated; blue, down-regulated. See Additional files 1 and 2 for further details. (C) Venn diagram of all significantly represented pathways on both the transcriptional and translational level. Eleven pathways were uniquely represented among differentially expressed genes, while 34 pathways were uniquely represented among differentially expressed proteins. Six pathways were jointly represented among differentially expressed molecules, and four of these were associated with energy metabolism (see Table 1 for further details).