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. 2008 Aug 27;79(6):1176–1182. doi: 10.1095/biolreprod.108.071217

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© 2008 by the Society for the Study of Reproduction, Inc.

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FIG. 4. — Expression of SOHLH1 and LHX8 proteins in 7-day-old ovaries. A–C) Anti-SOHLH1 antibodies (αSOHLH1) detected germ cell-specific transcription factor SOHLH1 in Postnatal Day 7 WT and Sohlh2^+/– ovaries, as shown by brownish cytoplasmic staining of primordial follicles (PF), primary follicles (PrF), and secondary follicles (SF). SOHLH1 protein was not significantly expressed in Sohlh2^–/– ovaries. D–F) Antibodies against LHX8 (αLHX8) localize LHX8 protein within the oocyte nuclei of PF, PrF, and SF in 7-day-old WT and Sohlh2^+/– ovaries but not in Sohlh2^–/– ovaries. Bar = 100 μm.