FIG. 5.

Misexpression of oocyte-specific genes in Sohlh2^–/– newborn ovaries. A–P) Expression of select genes in WT and Sohlh2^–/– newborn ovaries was assessed by real-time PCR: Sohlh2 (A), Sohlh1 (B), Nobox (C), Figla (D), Pou5f1 (E), Lhx8 (F), Gdf9 (G), Kitl (H), Zp1 (I), Zp2 (J), Zp3 (K), Stra8 (L), Kit (M), Oosp1 (N), Nlrp14 (O), and H1foo (P). Three independent pools of ovaries were used for each experiment, and each experiment was repeated in triplicate. Data are normalized to Gapdh expression and are given as the mean relative quantity (compared with WT), with error bars representing the SEM. Student t-test was used to calculate P values. *, P < 0.001. Q) RNA expression of selected genes in WT, Sohlh2^+/–, and Sohlh2^–/– newborn ovaries using semiquantitative RT-PCR.