FIG. 6.

In situ hybridization of Adm and its receptor components during the peri-implantation phase. In situ hybridization of the uterus at Days E0.5, E2.5, and E5.5 of pregnancy reveals that Adm, Calcrl, and Ramp2 are highly upregulated in uterine luminal epithelial cells. By Day E2.5 the expression of all genes becomes apparent in the stroma and glandular epithelium. Inset images at Day E2.5 show sense control probes. At Day E5.5, the expression of Adm is very strong in the first few cell layers surrounding the implanted conceptus, whereas the expression of Calcrl and Ramp2 is mostly localized to endothelial cell cords of the decidua. Scale bars are applicable to all images within a column. Insets were imaged with 2.5× objective.