Fig. 5.

Effect of different citrate salts on PEI-mediated transient transfection in CHO cells. CHO DXB11 cells were plated onto the wells of a six well plate in DMEM with 10% FBS. The following day, the media of each well was replaced with Medium A supplemented with 6 mM l-glutamine and Trace Elements A & B (Mediatech Inc.) and the indicated citrate salt, or iron (III) chloride, was added at a concentration of 50 μM. Cells were transfected with pEGFP-N1 as detailed in “Materials and methods”. After 48 h, cells were examined under a fluorescent microscope to determine expression of GFP. The experiment was performed three times with similar results