Fig. 8.
Expression of an IgG in Medium A with various iron carriers. CHO-S cells were seeded at a density of 2 × 106 cells/mL in 30 mL Medium A supplemented with 6 mM l-glutamine, Trace Elements A & B, and the indicated iron carrier at 50 μM (transferrin concentration was 20 mg/L). Cells were transfected with plasmids encoding the heavy and light chain of an IgG on separate plasmids (1:1 Light:Heavy chain ratio) as detailed in “Materials and methods”. At 4 and 8 days post transfection, samples were taken and analyzed by ELISA to determine antibody concentration in the media