Figure 6.
EPR Spectra of Fe(II)-HppE·nitrosyl complex with (R)-[P17O3]HPP (13-R). Black dotted line: (R)-[P16O3]HPP; Blue solid line: (R)-[P17O3]HPP. Instrumental parameters are the same as shown in Figure 5. Spectra are aligned to give approximately the same number of integrated spins of the E/D = 0.066 species. The minor resonance at g = 4.07 in the spectrum of the (R)-[P17O3]HPP (13-R) containing sample is due to a minor amount of denatured enzyme.2