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The assay for detecting anti-citrullinated type II collagen antibodies was performed in 130 serum samples from patients with rheumatoid arthritis (RA) and 40 serum samples from healthy donors. The transverse line shows the cutoff value. For the positive sera from RA patients, an inhibition assay was performed using citrullinated type II collagen as antigen. O.D., OD490 value
