Figure 5. Validation of microarray results by real-time PCR.

Expression of A) glycoprotein hormone alpha chain precursor (CGA), B) transthyretin (TTR), C) μ-Crystallin (CRYM), D) heat shock 70 KDa protein (HSPA2), E) zinc finger and BTB domain containing 16 (ZBTB16), F) growth arrest and DNA-damage-inducible, beta (GADD45B), G) vasoactive intestinal peptide (VIP), and H) c-fos (FOS) were determined. Results were calculated in relative RNA amounts using β-actin (ACTB) as internal control and shown as mean fold-change to group with lowest expression value. n = 5−7/group, * p<0.05 by ANOVA followed by Tukey's test as posthoc pair-wise comparisons. Results are shown in mean±SEM.