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. 2009 Dec 2;4(12):e8139. doi: 10.1371/journal.pone.0008139

Figure 5. CO activation of LPS-induced stress kinases.

Figure 5

CO and LPS activated (A) p38 MAPK, (B) ERK1/2, and (C) Akt as measured by Western blotting. (D) Total p38 served as a loading control. CO and LPS effects for all three kinases were additive. THP-1 cells (2×106) were treated with or without LPS (1 µg/ml) in the presence or absence of CO gas (250 ppm) for 1 h. Densitometric analysis is plotted as fold change from control. Results shown are mean ± SEM of three independent experiments along with representative blots.