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. 2009 Dec 2;4(12):e8067. doi: 10.1371/journal.pone.0008067

Figure 1. HDF can maintain self-renewal of established human iPS cells.

Figure 1

A. Images of iPS cells maintained on each fibroblast at passage number 10 and 19. Bars indicate 200 µm. B. 201B7 iPS cells at passage number 19 were plated on each feeder, and incubated for 6 days. The graph shows the percentage of TRA-1-60 positive colonies. Three individual assays were performed. Error bars indicate standard deviation. C. The number of colonies was counted and compared with the results of SNL feeder. This graph showed the average of three independent experiments. Error bars mean standard deviation. D. RT-PCR of undifferentiated ES cell markers. 201B7 iPS cells maintained on each HDF over 100 days were lysed, and their total RNAs were purified. One microgram of RNA sample was used for cDNA synthesis. qPCR was performed with the primers for endogenous OCT3/4, endogenous SOX2, NANOG and G3PDH. Data were normalized with the value of G3PDH. The graphs showed the average of three experiments. Error bars indicate standard deviation.