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. 2009 Dec 4;4(12):e8178. doi: 10.1371/journal.pone.0008178

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Wussow et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Scheme of the generation of pHJO variants with mini-F sequences (green) releasable by intrinsic genome replication processes. Upon mini-F transposition in E. coli, the vector sequences were shifted within the BAC-cloned HJO genome (pHJO) from the unique AvrII site of the US region (reverse with respect to UL, regarding the defined VZV prototype genome organization) into ORF62, ORF71, or directly into genomic terminal junction of VZV, to generate pHJOF62, pHJOF71, or pHJOFpac, respectively. Virus reconstitution from the pHJO variants yielded vector-free progeny rHJO-F^-.