Fig. 2.

Cancers remaining after therapeutic ICI treatment are associated with nonhypoplastic epithelium. (A) (Left) Low-magnification images of the two cancers [one cervical (Upper) and the other vaginal (Lower)] that persisted in 2 of 13 mice treated with ICI for 1 month. Black and gray arrowheads point to cancers and cancer-associated epithelia, respectively. (Scale bar, 100 μm.) (Right) High-magnification images of cervical (Upper) or vaginal (Lower) epithelium that was immediately juxtaposed to these two cancers. Note the nonhypoplastic (i.e., thick) epithelium akin to what is seen in female mice in normal estrus (see Fig. 1Dv). This is in stark contrast to the hypoplastic epithelia found elsewhere in the reproductive tracts of these same ICI-treated mice (Insets), as well as in the other ICI-treated, cancer-free mice (see Fig. 1Cv). White arrowheads point to dysplastic cells. Black lines delineate the basement membrane. (Scale bar, 50 μm.) (B) Cancers remaining in ICI-treated mice express ERα. Tissues were stained for ERα (red) and K14 (green), an epithelial cell marker. Arrowheads and arrows point to cancers and cancer-associated epithelia, respectively. (Scale bar, 50 μm.)