Fig. 5.
CD44, CEA, PCLP, and MCAM are candidate E-selectin ligands on PCa PC-3 cells. (A) Western blots of PC-3 cell transfectant protein were probed with anti-sLeX mAb HECA-452, E-selectin/Fc, anti-CEA, anti-CD44, anti-PSGL-1, anti-PCLP, anti-MCAM, or anti-β-actin. KG1a (HPC) and LS174T colon carcinoma (CCa) cell lysates were used as controls for CD44, CEA, and PCLP detection, and Malme-3M melanoma cells (Mel) cell lysate were used as control for MCAM detection. (B) Anti-CD44, (C) CEA, (D) PCLP, and (E) MCAM immunoprecipitates were blotted with HECA-452 and E-selectin/Fc, and with either anti-CD44, anti-CEA, anti-PCLP, or anti-MCAM. Immunoblotting with isotype controls did not show specific staining activity.