Fig. 3.

Adoptive transfer of CD4⁺foxp3⁺ Treg cells restored intestinal IgA in B6.TCRβ×δ^−/− mice. CD4⁺ foxp3⁺ Treg cells and CD4⁺ foxp3⁻ T cells from B6.GFP-foxp3 mice were transferred into B6.TCRβ×δ^−/− mice at 1 × 10⁶/mouse. (A) LP IgA⁺ B cells were analyzed by flow cytometry at day 21. (B) B cell numbers from four mice of each group. *, P ≤ 0.05 compared to the recipient mice of CD4⁺ foxp3⁻ T cells. (C and D) Stool pellets were collected at days 0, 4, 7, 14, and 21. Total IgA (C) and anti-CBir1 IgA (D) were assessed by ELISA. *, P ≤ 0.05 compared to foxp3⁻ T cell group.