Figure 3.

Immunohistochemical detection of PAR and nitrotyrosine in the gut of mice subjected to HS and resuscitation. Guts were removed from PARP^+/+ (A and C) and PARP^−/− (B and D) mice after HS and resuscitation, and tissue sections were stained for PAR as described in Materials and Methods. PAR and nitrotyrosine have been detected in the epithelial cells with strongest immunopositivity at the tip of the villi. Several stromal cells also displayed PAR positivity. No immunopositivity for PAR, reduced nitrotyrosine staining, and markedly maintained intestinal morphology were detected in the gut of, PARP^−/− mice (B and D). (Magnification ×400.)