Fig. 2.

Small size of Df[dilp1–5] homozygotes is due to reduced cell size and cell number. (A–E) Cell number and cell size in dissected wings. (A and B) Photos of dissected wings from (A) adult female, w¹¹¹⁸ control or (B) Df[dilp1–5] homozygote. (C) The overall wing area is smaller in Df[dilp1–5] homozygotes than in w¹¹¹⁸ controls. (D and E) Measurements of cell number and cell size in w¹¹¹⁸ or Df[dilp1–5] homozygotes. Both decreased in Df[dilp1–5] mutants. n = 5 for C–E. (F–K) Fat body cell size is reduced in Df[dilp1–5] animals. Photos of larval fat bodies from wandering third-instar larvae stained with phalloidin (red) and DAPI (blue) to reveal nuclei. [×20 (F, G, and H); ×40 (I, J, and K).] Genotypes: (F and I) Control parental line d02657, (G and J) Df[dilp1–5]/Df[dilp1–5], (H,K) hsGAL4>UASdilp2; Df[dilp1–5]/Df[dilp1–5]. (L) Quantitation of fat body cell size was carried out with ImageJ software. The cell size of Df[dilp1–5] homozygotes was approximately 80% of the parental control. Error bars indicate standard error.