Fig. 4.

Df[dilp1–5] homozygotes induce starvation responses. (A) Total body triglyceride level and total body protein from w¹¹¹⁸ or Df[dilp1–5] adult male flies were measured; data shows the triglyceride levels normalized to the total protein level. Levels in Df[dilp1–5] animals were lower than the control. Normalized levels: w¹¹¹⁸, 0.204 ± 0.022, n = 3; Df[dilp1–5], 0.090 ± 0.014, n = 3. Error bars indicate standard error. (B–E) Df[dilp1–5] homozygotes induce fat body autophagy, while actively eating. Lysotracker staining (red) of dissected fat bodies from third-instar larvae. Hoechst 33342 (blue) reveals nuclei. For (B, C, and E), larvae were actively eating, burrowed in the food and had full guts. Genotypes: (B) Control parental line d02657; (C) Df[dilp1–5]/Df[dilp1–5]; (D) Control parental line d02657; burrowed third-instar larva removed from food and starved for 3 h in the presence of water (E) hsGAL4>UASdilp2; Df[dilp1–5]/Df[dilp1–5]. Feeding Df[dilp1–5] homozygotes (C) resembled starved control animals (D).