Fig. 5.

Diabetic-like manifestations of Df[dilp1–5] homozygotes. (A) Df[dilp1–5] homozygotes show decreased metabolic rates. The overall metabolic rates of w¹¹¹⁸, Df[dilp1–5] or Df[dilp6] third-instar larvae, as indicated, were measured as the resting heat production normalized to body dry mass. Heat production rates were: w¹¹¹⁸, 44.89 ± 2.02, n = 18; Df[dilp1–5], 35.61 ± 2.14, n = 16; Df[dilp6], 46.46 ± 2.08, n = 17. Data represents mean ± standard error. (B and C) Circulating sugar levels are elevated in Df[dilp1–5] homozygotes. Sugar levels (trehalose + glucose) were determined in hemolymph extracted from (B) adult males, 3–5 days after eclosion, (n = 3) or (C) early or late third-instar larvae, as indicated. Levels are indicated for negative controls: w¹¹¹⁸ and parental line d02657; for experimental samples: Df[dilp1–5]/Df[dilp1–5], and hsGAL4>UASdilp2; Df[dilp1–5]/Df[dilp1–5]; (Rescue) and for the positive control: dilp2-GAL4>UAS-rpr (IPC ablation). Larval hemolymph was collected from 10–15 animals and pooled for each genotype (SI Experimental Procedures); a minimum of five replicates was used for each bar shown in the graph. The circulating sugar levels of Df[dilp1–5] homozygotes were higher than controls in early and late third-instar larvae. Levels were lowered by ubiquitous expression of DILP2. Sugar levels were highest in animals in which IPCs had been ablated (dilp2GAL4>UASrpr). Error bars indicate standard error.