Figure 3. Percentage cytotoxicity of splenocytes isolated from various groups of immunized and control BALB/C mice.

The splenocytes (Effector cells) were co-cultured with parasitized macrophages (Target cells) and cytotoxicity was measured as LDH release by spectrophotometric analysis. Statistically significant difference was seen in all the vaccinated groups when compared with controls (p<0.01). The results presented here are the mean values obtained from LDH release assay performed in triplicates.