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. 2009 Aug 18;58(12):2880–2892. doi: 10.2337/db09-0393

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© 2009 American Diabetes Association

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 5. — cGK-Tg mice exhibit giant mitochondria in the skeletal muscle, associated with higher oxygen consumption and fat oxidation. Mice were subjected to respiratory gas analysis after fed on high-fat diet. Total DNA and RNA were extracted from the brown adipose tissue and the quadriceps, and quantitative PCR analysis was performed. A: Oxygen consumption on standard diet (n = 6). ▩, wild type; ■, cGK-Tg. B: Mean oxygen consumption for 24 h on standard or high-fat diet. C: Rectal temperature on standard or high-fat diet (n = 12). D: Mean respiratory quotient for 24 h on standard or high-fat diet (n = 6). E: Mean fat oxidation estimated from the respiratory gas analysis for 24 h on standard or high-fat diet (n = 6). F: Mitochondrial DNA copy number estimated from quantification of mitochondrial and nuclear genome (n = 8). G: Expressions of genes encoding PGC-1α and UCP1 in the brown adipose tissue. The values were standardized to those for the control (wild-type [Wt] mice fed on standard diet) in either group (n = 12). H: Expressions of the genes involved in mitochondrial regulation or fatty acid catabolism in the skeletal muscle (n = 12). Standard diet: □, wild type (control); ▨, cGK-Tg. High-fat diet: ▩, wild type; ■, cGK-Tg. I: Electron microscopic analysis of muscle mitochondria of high-fat–fed cGK-Tg mice. *P < 0.05; **P < 0.01 vs. wild-type mice on the same feeding condition.

FIG. 5. — cGK-Tg mice exhibit giant mitochondria in the skeletal muscle, associated with higher oxygen consumption and fat oxidation. Mice were subjected to respiratory gas analysis after fed on high-fat diet. Total DNA and RNA were extracted from the brown adipose tissue and the quadriceps, and quantitative PCR analysis was performed. A: Oxygen consumption on standard diet (n = 6). ▩, wild type; ■, cGK-Tg. B: Mean oxygen consumption for 24 h on standard or high-fat diet. C: Rectal temperature on standard or high-fat diet (n = 12). D: Mean respiratory quotient for 24 h on standard or high-fat diet (n = 6). E: Mean fat oxidation estimated from the respiratory gas analysis for 24 h on standard or high-fat diet (n = 6). F: Mitochondrial DNA copy number estimated from quantification of mitochondrial and nuclear genome (n = 8). G: Expressions of genes encoding PGC-1α and UCP1 in the brown adipose tissue. The values were standardized to those for the control (wild-type [Wt] mice fed on standard diet) in either group (n = 12). H: Expressions of the genes involved in mitochondrial regulation or fatty acid catabolism in the skeletal muscle (n = 12). Standard diet: □, wild type (control); ▨, cGK-Tg. High-fat diet: ▩, wild type; ■, cGK-Tg. I: Electron microscopic analysis of muscle mitochondria of high-fat–fed cGK-Tg mice. *P < 0.05; **P < 0.01 vs. wild-type mice on the same feeding condition.