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. 2009 Aug 20;58(12):2910–2919. doi: 10.2337/db08-0506

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© 2009 American Diabetes Association

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 4. — Effects of PD153035 on adipocyte morphology and activation and migration of macrophages in adipocyte tissue. A: Histological sections of retroperitoneal fat pads from control, HFD, and HFDPD after 1 or 14 days, 50-μm scale bar for all pictures. B: Quantification of adipocyte size. About 100 cells were measured in each group, and the average adipocyte was calculated. C: Representative immunohistochemical staining of WAT using the specific macrophage marker F4/80+. D: F4/80-positive cells (+cells/total cells) of all above groups. Data are presented as means ± SE from six mice per group, *P < 0.05 vs. control group and #P < 0.05 vs. HFD. E: Bar graph indicates the number of THP1 cells that migrate from the top to the bottom level of the Boyden blindwell chamber stimulated or not with chemotatic agent RANTES. *P < 0.05 vs. DMSO alone; #P < 0.05 vs. DMSO/RANTES. Values represent the average of five different assays. (A high-quality color digital representation of this figure is available in the online issue.)