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Phospho–GSK-3β and total GSK-3β in total homogenates and subcellular fractions of the myocardium. A: Total tissue homogenate. B: Cytosol and mitochondrial fractions. Blots for β-actin and prohibitin are cytosolic and mitochondrial marker proteins, respectively, and also serve as loading controls. *P < 0.05 vs. LETO. †P < 0.05 vs. OLETF. n = 4–6. O+TUD, OLETF treated with TUDCA.
