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Ca2+-induced mPTP opening. A: Typical recording of mPTP opening in a sample of isolated mitochondria. B: Summarized data of Ca2+ load required to induce mPTP opening are presented for each group. *P < 0.05 vs. LETO; †P < 0.05 vs. OLETF. n = 3. C: Relationship between the amounts of Ca2+ required for mPTP opening and phosphorylation levels of GSK-3β in mitochondria at reperfusion. ●, LETO; ○, OLETF; △, OLETF+TUDCA. O+TUD, OLETF treated with TUDCA.
